全文获取类型
收费全文 | 8523篇 |
免费 | 1032篇 |
出版年
2021年 | 109篇 |
2019年 | 76篇 |
2018年 | 92篇 |
2017年 | 82篇 |
2016年 | 151篇 |
2015年 | 230篇 |
2014年 | 268篇 |
2013年 | 324篇 |
2012年 | 373篇 |
2011年 | 402篇 |
2010年 | 263篇 |
2009年 | 217篇 |
2008年 | 316篇 |
2007年 | 382篇 |
2006年 | 320篇 |
2005年 | 312篇 |
2004年 | 276篇 |
2003年 | 260篇 |
2002年 | 259篇 |
2001年 | 304篇 |
2000年 | 286篇 |
1999年 | 229篇 |
1998年 | 112篇 |
1997年 | 110篇 |
1996年 | 86篇 |
1995年 | 94篇 |
1994年 | 96篇 |
1993年 | 88篇 |
1992年 | 182篇 |
1991年 | 161篇 |
1990年 | 161篇 |
1989年 | 179篇 |
1988年 | 147篇 |
1987年 | 169篇 |
1986年 | 157篇 |
1985年 | 165篇 |
1984年 | 136篇 |
1983年 | 120篇 |
1982年 | 97篇 |
1981年 | 96篇 |
1980年 | 77篇 |
1979年 | 115篇 |
1978年 | 92篇 |
1977年 | 116篇 |
1976年 | 90篇 |
1974年 | 119篇 |
1973年 | 86篇 |
1972年 | 77篇 |
1971年 | 91篇 |
1969年 | 81篇 |
排序方式: 共有9555条查询结果,搜索用时 578 毫秒
71.
72.
R. Ray D. Rincon-Limas R.A. Wright S.N. Davis J.R. Lupski P.I. Patel 《Nucleic acids research》1990,18(16):4958
73.
Raymond R. Wilson Jr Joseph F. Siebenaller Bonnie J. Davis 《Biochemical Systematics and Ecology》1990,18(7-8):565-572
The heart-type lactate dehydrogenase (LDH-B4) homologs of two species of Nezumia and eight species of Coryphaenoides are isomobile on two commonly used electrophoretic buffer systems. To test the hypothesis that the homologs possess the same primary structures, the allozymes from N. bairdii and four species of Coryphaenoides were purified by affinity chromatography on an oxamate aminohexyl Sepharose column and digested with trypsin. The resulting peptide mixtures were then mapped using reversed-phase high-performance liquid chromatography. The peptide maps of the enzyme homologs indicate that the overall similarity of the homologs is high, but unique peptides in each species indicate that the allozymes are not identical in primary structure. 相似文献
74.
J D Robinson M Guerra R L Davis M Steinberg 《Nauchnye doklady vysshe? shkoly. Biologicheskie nauki》1990,(6):97-106
To help characterize the Na,K-ATPase active site with enzyme incorporated into phospholipid vesicles, the activities with alternative substrates were compared, 22Na/Na-transport was equivalent with ATP, CTP, carbamylphosphate and acetylphosphate, but slower with CTP, 3-O-methylfluoresceinphosphate (3-O-MFP), nitrophenylphosphate and umbelliferonephosphate. It indicates a slower rate of formation of phosphorylating enzyme complex in conformation position of E1 (E1P) when the second group of substrates is bound with enzyme active center. 22Na/K-transport was half as effective with CTP as with ATP and was far slower with the other substrates. It indicates a more stringent selectivity at the low-affinity site of enzyme in conformation E2 that accelerates the slow step of this transport mode. Although enzyme modification with fluoresceinisothiocyanate blocks the high-affinity site to ATP, the K-phosphatase reaction catalyzed by E2 is retained, even with a substrate, 3-O-MFP, that binds to the adenine pocket. Dimethylsulfoxide inhibits hydrolysis of the nucleotides and of the carboxylic phosphate substrates of the K-phosphatase reaction, but stimulates hydrolysis of the phenolic phosphate substrates (nitrophenylphosphate and umbelliferone phosphate) which normally are hydrolyzed more slowly than the other substrates. On the basis of these data the authors propose the model of Na,K-ATPase active center. 相似文献
75.
76.
77.
Recognition of the P1 plasmid centromere analog involves binding of the ParB protein and is modified by a specific host factor. 总被引:27,自引:4,他引:23 下载免费PDF全文
The P1 plasmid partition system is responsible for segregation of daughter plasmids during division of the Escherichia coli host cell. The P1-encoded elements consist of two essential proteins, ParA and ParB, and the cis-acting incB region. The incB region determines partition-mediated incompatibility and contains the centromere-like site parS. We have isolated and purified the two proteins. ParB binds specifically to the incB region in vitro. DNase I footprinting assays place a strong binding site over the 35-bp parS sequence previously shown to be sufficient for partition when the Par proteins are supplied in trans. A weaker site lies within the incB region in sequences that are important for specifying incompatibility, but are not essential for partition. Gel band retardation assays show that a host factor binds specifically to the incB sequence. The factor strongly stimulates binding of ParB. Cutting the region at a site between the two ParB binding sites yields two fragments that can bind ParB but not host factor. Thus, information for host-factor binding lies in the region determining the specificity of plasmid incompatibility. The roles of parB and the host factor in partition and the specificity of plasmid incompatibility are discussed. 相似文献
78.
Characterization of an antigenically related family of cell-type specific proteins implicated in slug migration in Dictyostelium discoideum 总被引:1,自引:0,他引:1
Stephen Alexander Elizabeth Smith Loralie Davis rew Gooley Suzanne B. Por Lois Browne Keith L. Williams 《Differentiation; research in biological diversity》1988,38(2):82-90
The monoclonal antibody MUD50 recognizes a group of developmentally regulated proteins, which are almost exclusively expressed by prespore cells in developing aggregates of Dictyostelium discoideum. Some of these antigens are integrally associated with the cell membrane, as assessed by physical and detergent-fractionation procedures. The MUD50-reactive proteins are glycosylated and some are phosphorylated. Post-translational modification is the common antigenic feature that is recognized by the MUD50 antibody in these cell-type-specific proteins. A glycosylation-defective mutant, DL118, (modB) does not express the MUD50 epitope, but does express the MUD52 epitope, which is found on a different group of glycoproteins. Therefore, we conclude that MUD50 recognizes a particular carbohydrate epitope on a restricted group of proteins. These proteins are structurally diverse, but are apparently involved in the maintenance of structure and movement of the multicellular D. discoideum slug. 相似文献
79.
Close association of a DNA replication origin and an ARS element on chromosome III of the yeast, Saccharomyces cerevisiae. 总被引:53,自引:13,他引:40 下载免费PDF全文
Two dimensional gel electrophoretic techniques were used to locate all functional DNA replication origins in a 22.5 kb stretch of yeast chromosome III. Only one origin was detected, and that origin is located within several hundred bp of an ARS element. 相似文献
80.
Charles E. Stager Dr. James R. Davis Mario N. Saccomani Corpus O. Ortigoza Reuben D. Wende James W. Raleigh 《Current microbiology》1988,17(5):243-247
Two-hundred and fifteen isolates ofMycobacterium tuberculosis were evaluated with the BACTEC 460 radiometric method for susceptibility to isoniazid, rifampin, ethambutol, and streptomycin (SM); a revised protocol for inoculum preparation was used. Fresh clinical isolates were subcultured into 7H9 broth and then photometrically adjusted to the equivalent of a 0.5 McFarland standard, one-half the recommended inoculum density. This method produced an overall 98.3% correlation with a conventional agar method. The sensitivity of this procedure was good for all drugs tested except for the lowest concentration of SM (2 g/ml). Specificity was excellent for all drugs tested. After repeat testing, only four discrepancies were found, yielding a 99.8% correlation between the two systems. The time required for susceptibility tests averaged 4.6 days. This method for inoculum preparation effectively minimized the number of susceptibility tests exceeding the threshold value before the fourth day of incubation. This allowed for definite trends of the growth index values to become established before interpretation of results. 相似文献